Dynamic Light Scattering Spectrophotometer DLS-8000 series
Measurement system for particle size and particle-size distribution using “dynamic light-scattering method” and absolute molecular weight, radius of gyration, and the second virial coefficient using and “static light-scattering method"
- Measured data
- Measures particle size and particle-size distribution based on the dynamic light-scattering method, and absolute molecular weight, radius of gyration, and the second viral coefficient based on the static light-scattering method.
- He-Ne laser, Ar laser, double laser (He-Ne, Ar) models are available.
- Adopts a liquid-immersion optical system, supporting high precision measurement for extremely weak scattering of nano order particles.
- Supports multimode analysis of concentrated polymer solutions, using a correlator providing up to 4,096 channels.
- Supports gel-state analysis in combination with the optional gel rotation cell.
|Particle sizing range|
|1.4 nm ～ 7000 nm (Ar)|
|3 nm ～ 7000 nm (He-Ne)|
|Translation diffusion coefficient||2×10-6 ～ 2×10-9 cm2sec-1|
|Molecular weight measurement range||3×102～ 2×107|
|Radius of gyration||20 ～ 1000 nm|
|Second virial coefficient||-1 ～ 20×10-4mol・cm3・g-2|
- Synthetic Polymer
Dilute polymer solution, latex, emulsion, micelle, sol, concentrated polymer solution, polymer melting solution
- Bio polymer
Protein, nucleic acid, virus, polysaccharide, liposome, vesicle, cell
- Inorganic material
Inorganic dilute polymer solution, ceramics, pigment, magnetic substance, metallic colloid
Particulates dispersed in a solution are normally subject to Brownian motion. The motion is slower with larger particles and faster with smaller particles. When laser light illuminates particles under the influence of Brownian motion, scattered light from the particles shows fluctuation corresponding to individual particles. The fluctuation is observed according to the pinhole type photon detection method so that particle size and particle size distributions are calculated.
Static Light Scattering Method is renowned as the convenient method to know absolute molecular weight. As a principle, molecular weight is calculated from absolute value of scattered light obtained by irradiated light into colloidal particle. To simplify, from the bigger particle gives the stronger scattering and the smaller particle gives weaker scattering. In reality, scattering intensity to be obtained depends upon concentration, too. So the plotting concentration on horizontal and Kc/R(θ), which is equivalent as a reciprocal number of scattering intensity, on vertical after measuring scattering intensities of variable concentrations is called Debye Plot.Since angle dependency of scattering intensity appears in the large molecular weight sample, measuring scattering intensity at variable angle(θ) provides more accuracy on molecular weight measurement and radium of gyration, which is yardstick of molecular dispersion.When measured at fixed angle, inputting expected radium of gyration, correcting as an angle dependent measurement, accuracy of molecular weight is improved.
|Light source|| He-Ne laser
|He-Ne laser + solid laser(10mW)（100mW)|
|Detector||Photomultiplier tube (photo counting method)|
|Cell||21φCylindrical cell, 12φCylindrical cell、５φmicro volume cell (option)|
|Cell Temperature|| 5～90℃ （Thermostatic circulating system ：Circulation bath - Optional)
90 ～ 160℃（Temp. control system：only available for high temp. spec）
|Angle range||5 ～ 160゜（Automatic/ Stepping motor drive）|
|Power supply||AC100V 300VA|
|Size（WDH）||440×700×570 mm||590×700×570 mm|
|Weight||Approx. 50 kg||Approx. 60 kg|
Micro volume sample can be measured (50μL～)
To be used in low-angle measurement at MW or microparticle measurement to minimize stray light.
Information related to anisotropy of component can be obtained by measuring and analysing scattering intensity of polarization component
Perfect sample filtration kit for dust removal-which is one of the biggest challenge in light scattering method. Solvent resistant-usable with many organic solvent (stand/syringe/filter is NOT included)
|Temp. display||0.05 ℃|
|Temp. control system||±0.01 ℃|
|Weight||Approx. 24 kg|
|Power supply||1.1 kW|
- Measured data
|Zeta-potential & Particle size Analyzer ELSZ-2000 series|