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Dynamic Light Scattering Spectrophotometer DLS-8000 series

Measurement system for particle size and particle-size distribution using “dynamic light-scattering method” and absolute molecular weight, radius of gyration, and the second virial coefficient using and “static light-scattering method"


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  • Measures particle size and particle-size distribution based on the dynamic light-scattering method, and absolute molecular weight, radius of gyration, and the second viral coefficient based on the static light-scattering method.
  • He-Ne laser, Ar laser, double laser (He-Ne, Ar) models are available.
  • Adopts a liquid-immersion optical system, supporting high precision measurement for extremely weak scattering of nano order particles.
  • Supports multimode analysis of concentrated polymer solutions, using a correlator providing up to 4,096 channels.
  • Supports gel-state analysis in combination with the optional gel rotation cell.


Measuring Item
Particle sizing range
 1.4 nm ~ 7000 nm (Ar)
 3 nm ~ 7000 nm (He-Ne)
Translation diffusion coefficient  2×10-6 ~ 2×10-9 cm2sec-1
Molecular weight measurement range  3×102~ 2×107
Radius of gyration  20 ~ 1000 nm
Second virial coefficient  -1 ~ 20×10-4mol・cm3・g-2


Measuring Object
  • Synthetic Polymer
    Dilute polymer solution, latex, emulsion, micelle, sol, concentrated polymer solution, polymer melting solution
  • Bio polymer
    Protein, nucleic acid, virus, polysaccharide, liposome, vesicle, cell
  • Inorganic material
    Inorganic dilute polymer solution, ceramics, pigment, magnetic substance, metallic colloid




Principle of particle sizing: Dynamic Light Scattering Method (Photon Correlation Method)

Particulates dispersed in a solution are normally subject to Brownian motion. The motion is slower with larger particles and faster with smaller particles. When laser light illuminates particles under the influence of Brownian motion, scattered light from the particles shows fluctuation corresponding to individual particles. The fluctuation is observed according to the pinhole type photon detection method so that particle size and particle size distributions are calculated.


Analysis procedure

Analysis procedure


Molecular Weight: Static Light Scattering Method

Static Light Scattering Method is renowned as the convenient method to know absolute molecular weight. As a principle, molecular weight is calculated from absolute value of scattered light obtained by irradiated light into colloidal particle. To simplify, from the bigger particle gives the stronger scattering and the smaller particle gives weaker scattering. In reality, scattering intensity to be obtained depends upon concentration, too. So the plotting concentration on horizontal and Kc/R(θ), which is equivalent as a reciprocal number of scattering intensity, on vertical after measuring scattering intensities of variable concentrations is called Debye Plot.Since angle dependency of scattering intensity appears in the large molecular weight sample, measuring scattering intensity at variable angle(θ) provides more accuracy on molecular weight measurement and radium of gyration, which is yardstick of molecular dispersion.When measured at fixed angle, inputting expected radium of gyration, correcting as an angle dependent measurement, accuracy of molecular weight is improved.



  DLS-8000HL/HH DLS-8000DL/DH
Light source  He-Ne laser
 He-Ne laser + solid laser(10mW)(100mW)
Detector  Photomultiplier tube (photo counting method)
Cell  21φCylindrical cell, 12φCylindrical cell、5φmicro volume cell (option)
Cell Temperature  5~90℃ (Thermostatic circulating system :Circulation bath - Optional)
 90 ~ 160℃(Temp. control system:only available for high temp. spec)
Angle range  5 ~ 160゜(Automatic/ Stepping motor drive)
Accuracy  ±0.1°
Power supply  AC100V 300VA
Size(WDH)  440×700×570 mm  590×700×570 mm
Weight  Approx. 50 kg  Approx. 60 kg


Measured data

Autocorrelation function

Autocorrelation function


Distribution of Particle size

Distribution of Particle size



φ5micro volume cell /cell holder

Micro volume sample can be measured (50μL~)


φ30micro volume cell /cell holder

To be used in low-angle measurement at MW or microparticle measurement to minimize stray light.


Polarizing element(detector/irradiater)

Information related to anisotropy of component can be obtained by measuring and analysing scattering intensity of polarization component


Differential Refractometer(dn/dc)DRM-3000

Differential Refractometer(dn/dc)DRM-3000


Sample filtration kit

Perfect sample filtration kit for dust removal-which is one of the biggest challenge in light scattering method. Solvent resistant-usable with many organic solvent (stand/syringe/filter is NOT included)


Circulation bath


Temperature range
 -20~150 ℃
Temp. display  0.05 ℃
Temp. control system  ±0.01 ℃
Power supply  100V:50/60Hz
Size(WDH)  200×400×557 mm
Weight  Approx. 24 kg
Power supply  1.1 kW


Related Information

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