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Zeta-potential & Particle size Analyzer ELSZneo NEW

ELSZneo is the high-end model of the ELSZ series. This instrument measures zeta potential and particle size of both diluted and concentrated solutions, and molecular weight of a polymer. As new features, ELSZneo provides a multi-angle measurement to improve the separability of particle size distribution, particle concentration measurement, microrheology measurement, and gel network analysis.
The zeta potential flat surface cell adopted a newly developed coating which enables measurement in a high salt concentration such as physiological saline. With the brand-new flat surface cell and ultra-micro volume cell that can measure particle size with a 3 μL of sample, ELSZneo is expanding the potential in the life science field.


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  • Particle size and zeta potential measurement in a wide concentration range from diluted to concentrated solution (~ 40%)
  • Multi-angle measurement to carry out particle size distribution with higher separability
  • Zeta potential measurement of solid surface in high salt concentration
  • Particle concentration analysis using Static Light Scattering     
  • Microrheology measurement using Dynamic Light Scattering
  • Gel network and gel heterogeneity analysis by measuring intensity of scattered light and diffusion coefficient of sample at multiple points 
  • Standard flow cell which can be used for two types of measurement (Particle size and zeta potential) without replacing samples
  • Wide range of measurement temperature from0to 90℃
  • Temperature gradient function which enables denaturation (melting temperature) analysis for proteins
  • Electrophoretic mobility measurement and plot analysis for accurate zeta potential measurement
  • Band-pass filter (option)
Measurement Items
  • Particle Size Distribution
  • Zeta Potential
  • Molecular Weight
  • Microrheology
  • Particle Concentration
  • Structural Analysis of Gel Networks
Measurement range
Zeta Potential  No effective limitations
Mobility  -2×10 -5 ~ 2×10 -5 cm2/V・s
Particle Size  0.6 nm ~ 10000 nm*1
 (display range:0.1-106nm)
Molecular Weight  340 ~ 2×107

 1:Minimum value with histogram analysis : 0.2 nm

●Measure conditions

Temperature  0 ~ 90℃
Concentration  Particle Size : 0.00001 % (0.1ppm) ~ 40 % *2
 Zeta Potential : 0.001%~40%

*2:(Latex112nm: 0.00001 ~ 10%、Taurocholic acid: ~ 40%)



Best suitable for basic and applied research of particle characterization in the field of surface chemical, inorganic, semiconductor, polymer, biotechnology, pharmaceutical and medical as well as surface research of film and flat state sample.

  • New functional material
    Fuel cell(Carbon nanotube,  Fullerene, Function film, Catalyst, Nano-metal)
    Bionanotechnology ( Nano capsule, Dendrimer, Drug Delivery System(DDS)),  Nanobubble
    Biocompatible material
  • Ceramic and paints
    Ceramic(Silica, Alumina, TiO2)
    Surface modification, dispersion control of inorganic sol
    Dispersibility, durability and shelf life control of ink, carbon black and organic pigment
    Slurry state sample
    Color filter
    Flocculation research

  • Semiconductor
    Alien objects research on silicon wafer
    Interaction research of abrasive/additive on wafer surface
    CMP slurry
  • Polymer and Chemical
    Emulsion dispersion stability to extend product shelf life
    Surface modification of latex
    Function research of polyelectrolyte
    Process control of paper production and Pulp additive research
  • Pharmaceutical and food 
    Emulsion dispersion stability to extend product shelf life
    Dispersibility control of liposome and vesicle
    Functionality of surfactant(Micelle)
    Viruses, Virus-like particles(VLP)



Principle of particle sizing: Dynamic Light Scattering Method (Photon Correlation Method)

Particulates dispersed in a solution are normally subject to Brownian motion. The motion is slower with larger particles and faster with smaller particles. When laser light illuminates particles under the influence of Brownian motion, scattered light from the particles shows fluctuation corresponding to individual particles. The fluctuation is observed according to the pinhole type photon detection method so that particle size and particle size distributions are calculated.


Analysis procedure


Principle of Zeta Potential Measurement: Electrophoretic Light Scattering Method (Laser Doppler Method)

In most cases, colloidal particles possess a positive or negative electrostatic charge. As electrical fields are applied to the particle dispersion, the particles migrate in oppositely charged directions. As particles are irradiated in migration, scattering light causes Doppler shift depending on electrophoretic mobility. This method is called Laser Doppler Method.


Advantage of Electro-Osmosis Measurement

Electro-osmosis is the liquid flow occurred inside cell upon zeta potential measurement. If cell wall is electrically charged, counter ion in medium migrates to cell wall.  This is the phenomenon that counter ion migrates to one electrode in the cell wall and to other electrode in the vicinity of cell center. By measuring apparent electrophoresis mobility and analyzing electro osmosis, it becomes possible to obtain correct stationary layer taking stained cell into consideration and to calculate correct zeta potential. (Mori-Okamoto equation)

<Mori-Okamoto equation >


z:Distance from cell center
Uobs(z):Apparent mobility at (z) inside cell
k=2a and 2b are respectively the horizontal and vertical length of square phase of cell. Here a>b.  
Up:True mobility of particle
U0:Average mobility at upper and bottom walls
⊿U0:Gap of mobility at upper and bottom walls

Electro-osmosis for multi component analysis

Apparent electro-osmosis measure at multiple points inside cell enables repeatability check of zeta potential and noise or peak determination.


Flat surface cell for solid sample

Flat surface cell is configured with box-like quartz cell with flat surface sample attached on it. Measure apparent electrophoresis mobility of monitoring particle at several positions in vertical inside cell and analyze mobility of electro osmosis on solid surface using electro osmosis profile obtained to calculate zeta potential.


Principle of zeta-potential measurement using high concentration cell


It used to be difficult to measure very condensed or colored sample due to multiple scattering or absorption effect. Currently standard cell of ELSZ series is able to measure wide concentration range of sample. Furthermore, zeta potential of very condensed sample can be measured by FST method*


Molecular Weight : Static Light Scattering Method.


Static Light Scattering Method is renowned as the convenient method to know absolute molecular weight. As a principle, molecular weight is calculated from absolute value of scattered light obtained by irradiated light into colloidal particle. To simplify, from the bigger particle gives the stronger scattering and the smaller particle gives weaker scattering. In reality, scattering intensity to be obtained depends upon concentration, too. So the plotting  concentration on horizontal and Kc/R(θ), which is equivalent as a reciprocal number of scattering intensity, on vertical after measuring scattering intensities of variable concentrations is called Debye Plot.  


Since angle dependence of scattering intensity appears in the large molecular weight sample, measuring scattering intensity at variable angle(θ) provides more accuracy on molecular weight measurement and radius of gyration, which is yardstick of molecular dispersion. When measured at fixed angle, inputting expected radium of gyration, correcting as an angle dependent measurement, accuracy of molecular weight is improved.


Second Virial Coefficient


Second Virial Coefficient expresses the degree of attraction and repulsion of monomers, giving the yardstick of affinity and crystallization against molecule of solvent.

  • Given A2 is positive, repulsion in molecules is big in high affinity good solvent, it exists steadily.
  • Given A2 is negative, affinity in molecules is big in low affinity poor solvent, it aggregates easily.
  • Given A2 is zero, the solvent is called theta solvent and its temperature is called theta temperature where attraction and repulsion are proportional, causing crystallization easily.


Measurement Items
  • Particle Size Distribution
  • Zeta Potential
  • Molecular Weight
  • Microrheology
  • Particle Concentration
  • Structural Analysis of Gel Networks


Principle  Particle size  Dynamic light scattering method
 Zeta potential  Electrophoretic light scattering method
    (Laser doppler method)
 Molecular weight  Static light scattering method
Optics  Particle size  Homodyne system
 Zeta potential  Heterodyne system
 Molecular weight  Homodyne system
Light source  Narrow-band laser diode
Detector  High sensitivity APD
Cell unit     Zeta potential/Particle size:Standard flow cell unit
   Particle size:Particle size cell unit
 Particle size/Molecular weight : Particle size multi-angle cell unit
Temperature  0 ~ 90℃ (with gradient function)
Power requirements  AC 100 - 240V, 50/60Hz, 250VA
Size(WDH)  330(W)×565(D)×245(H)mm
Weight  Approx. 22 kg
Standards    Particle size:      ISO 22412:2017 / JIS Z 8828:2019
   Zeta potential:   ISO 13099-2:2012/JIS Z 8836:2017


Multi-angle measurement for higher separability

Measurement and analysis from 3 angles - front, side, and back – provides a higher-resolution particle size distribution with higher separability.
Sample peaks that cannot be differentiated with measurement from 1 angle can be separated into individual peaks via measurement and analysis from 3 angles.



Particle concentration measurement

Static light scattering allows calculation of the particle concentration in solution.



Dynamic light scattering allows measurement of the viscoelasticity of “soft” structures such as polymers and proteins.



Renewed flat surface cell

Our flat surface cell has been upgraded to measure the surface zeta potential of flat samples. The cell’s newly developed coating to resist high salt concentrations allows measurement under highly saline conditions (a 154 mM NaCl solution), facilitating the evaluation of biocompatible materials.


A wide range of concentrations, from low to high, can be measured

The size and zeta potential of particles can be measured in solutions ranging from a 0.00001% dilute solution (0.1 ppm) to a solution concentrated up to 40%.


Zeta potential of printer inks

Zeta potential of ink liquid (Black)

Particle size of Thiamin(Vitamin B1) and Latex 10360 nm


Mixed sample of polystyrene latex


Measurement example with flat surface cell

Zeta potential of negatively charged glass surface (BLANK)
Zeta potential =-58.4 mV(1 mM NaCl solution)


Negatively charged glass surface being neutralized by positively charged CTAB
Zeta potential =+1.3 mV(1 mM NaCl solution containing 1×10-5 mol/l CTAB)


Positively charged status with the excessive CTAB adsorbed on glass surface  
Zeta potential =+35.5 mV(1 mM NaCl solution containing 1×10-4 mol/l CTAB )


Measurement example with micro volume disposable cell

Electro-osmosis plotting and zeta potential of polystyrene latex particles in 100 mM NaCl 


Molecular weight of BSA(4℃)


Molecular weight of F40(25℃)


Melting point (MP) of protein using temperature gradient function


Melting point(MP) of cellulose derivative


Surface zeta potential of contact lens


Surface zeta potential of human hair


Zeta potential flat surface cell unit/ Zeta potential small flat surface cell unit

Cell units for surface zeta potential measurement of flat samples and films Also for measurement under high salt concentration conditions

●The cell is easy to assemble
 Screw-less design

●A variety of simple coatings
 Which Customers can apply coatings by themselves

●Suitable for very small samples
 Samples 10×10 mm in size


Zeta potential micro volume disposable cell unit

A cell unit for zeta potential measurement with small sample quantities ( 130μL- )


Zeta potential high concentration cell unit

A cell unit for zeta potential measurement in concentrated suspensions


Zeta potential low permittivity cell unit

A cell unit for zeta potential measurement in a nonpolar solvent
Suitable for solvents with a dielectric constant lower than 10


Particle size ultra micro volume cell unit

A cell unit for particle size measurement with small sample quantities (3 μL-)粒子径超微量ガラスセルユニット

pH titrator(ELSZ-PT)

Changes in the size and zeta potential of particles with respect to pH or the concentration of an additive concentration can be measured automatically

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