Zeta-potential and Particle Size Analyzer

WithELSZneo,

Features

1. Feature 01 Highly reliable measurement results

   The reliability of particle size measurement results can be confirmed by reviewing the autocorrelation function of the raw data and the fitting residuals from the analysis. Particle size monitor plots also enable real-time observation of sample changes during measurement.　By visualizing raw data and sample behavior over time alongside analyzed results, ELSZneo delivers highly reliable measurement results with greater confidence.

   

2. Feature 02 Zeta potential (Electroosmotic flow)

   Accurate zeta potential measurements require analysis of the electroosmotic flow generated within the measurement cell.　During electrophoretic measurements in a closed cell, electroosmotic flow caused by the charged cell walls is unavoidable. By performing measurements at multiple positions within the cell and analyzing the stationary layer that is unaffected by electroosmotic flow, the true particle mobility can be determined.　In addition, multipoint measurements within the cell enable clear discrimination between true component peaks and noise peaks.

   

3. Feature 03 Measure particle size and zeta potential under biologically relevant condition

   The large dome-shaped electrode enables zeta potential measurements in high-salinity environments, such as physiological saline. Because zeta potential is highly dependent on ionic strength, it is important to measure zeta potential under the sample's actual solvent conditions.

   

4. Feature 04 Zeta potential (Flat plate cell)

   Using the optional flat plate cell, the zeta potential of planar samples such as films can be measured. Hair and other fiber-like samples can also be analyzed using a dedicated spacer kit for sample preparation.　This enables the evaluation of electrostatic interactions between particles and flat surfaces, such as pigments and printing paper, polishing particles and wafers, and hair and hair-treatment products.

   

5. Feature 05 Gel network structure

   Dynamic light scattering (DLS) measurements of gel samples can be used to analyze the mesh size and structural heterogeneity of the gel. Furthermore, by monitoring the time-dependent changes in scattering intensity and diffusion coefficient, the gelation process can be evaluated.

   

Use Cases

1. Challenge1 Need to measure under biologically relevant salt concentrations（e.g., exosomes).

   Evaluate biosamples under physiologically relevant conditions.
   Conventional zeta potential measurements often require dilution when using high-ionic-strength PBS buffers. In contrast, this system enables direct measurement of MSC-EVs in PBS without dilution. Because HEPES has a lower ionic strength than PBS, a higher absolute zeta potential was observed. Measurements under conditions that closely reflect the actual use environment enable a more accurate and realistic assessment of particle surface charge.

   

2. Challenge2 Need to measure interactions between solid surfaces and liquids.

   Need to measure interactions between solid surfaces and liquids.
   Flat plate zeta potential measurement allows for the evaluation of the surface conditions of flat samples such as glass, resin, and fibers. It is effective for evaluating electrostatic interactions with particles, comparing surface properties before and after coating, and analyzing correlations with wettability.

   

3. Challenge3 Need to measure the sample in its original concentration without dilution.

   Evaluate zeta potential under conditions that closely resemble the actual sample by minimizing dilution effects.
   For some samples, dilution can alter the dispersion state or the particle surface environment, potentially resulting in zeta potential values that differ from those of the original sample. By enabling measurements of highly concentrated samples, zeta potential can be evaluated under conditions that more closely reflect actual use environments.

   

4. Challenge4 Need higher particle size distribution resolution

   Achieve higher particle size resolution with multi-angle DLS.
   Combining data from multiple scattering angles improves particle size distribution resolution. This enables the differentiation of closely spaced particle size populations and the detection of larger particles that can be difficult to resolve with single-angle measurements.

   

5. Challenge5 Need to know the number of particles

   Quantify particle concentration over a wide range with SLS.
   Particle counting methods based on image analysis can be limited in the range of concentrations that can be measured. In contrast, the SLS method exhibits excellent linearity over a wide concentration range, enabling accurate particle concentration measurements, even for highly concentrated samples.

   

6. Challenge6 Need to quantify the mesh size within a gel

   Measure gel structures non-destructively and without contact.
   DLS-based gel mesh size measurements enable the evaluation of internal gel structures without damaging the sample. In polyacrylamide gels, changes in mesh size associated with variations in gel concentration can be quantified, providing valuable information for material design and property evaluation.

   

Video

• Zeta-potential and Particle Size Analyzer ELSZneo

Specifications

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